Table 1.
IC50 values of monomers and oligomers of catechin, epicatechin, and resveratrol, obtained from the control and enzymatic reactions after 2 h of reaction.
| Products and Standard. | Fe2+ Chelating Activity IC50 * (μM CE) |
Cu2+ Chelating Activity IC50 (μM CE) |
|---|---|---|
| Control reaction: | ||
| Catechin | 1484.3 ± 3.2 b | 889.8 ± 7.2 d |
| Epicatechin | 1633.9 ± 32.5 a | 1105.8 ± 36.9 c |
| Resveratrol | 1484.4 ± 42.9 b | 937.1 ± 25.8 d |
| Enzymatic reaction: | ||
| Catechin | 0.4 ± 0.02 g | 1.8 ± 0.02 g |
| Epicatechin | 0.5 ± 0.00 g | 1.7 ± 0.06 g |
| Resveratrol | 0.5 ± 0.02 g | 20.9 ± 0.3 g |
| Standard: | ||
| EDTA | 125.2 ± 1.4 e (μM EDTA) | 367 ± 6.1 f (μM EDTA) |
Values are mean ± SEM, n = 3. No significant differences in the values that share the same letter. * IC50 is the concentration of sample required to chelate 50% of the metallic ions. µM CE: µM of catechin equivalents (CE). The enzymatic reaction contained polyphenol compounds (PCs) at 1.5 mM concentration, 2.5 U/mL of peroxidase and 0.05% hydrogen peroxide (H2O2). In the control reaction, peroxidase was substituted by phosphate buffer solution. EDTA = ethylendiaminetetraacetic acid. Letters a–g represents the significant differences estimated by ANOVA followed by the Tukey test.