Figure 4.
Time profiles of l-lysine consumption and cadaverine synthesis by recombinant E. coli XL1-Blue strain harboring E. coli ldcC gene and H. alvei ldcC genes in shake flask scale whole cell bioconversion reactions. A recombinant E. coli strains seed inoculum (OD600 = 50) was prepared as whole cell biocatalysts for the conversion of 200 g/L of industrial grade l-lysine to cadaverine. Data represent mean values from three independent experiments with corresponding standard deviations. (Symbols: filled circle, l-lysine concentration; open circle, cadaverine concentration).