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. 2019 Jul 5;7(7):192. doi: 10.3390/microorganisms7070192

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Generating heterologous synthetic marker and reporter genes. (A) Linear maps of pRS-AsTEF1p-LacZ and pRS-AsTEF1p-ScRAD51-HYG3 plasmids. (B) The AsTEF1p-LacZ reporter gene and the HYG3 resistance marker were tested in Saccharomyces cerevisiae BY4741. The presence of an active β-galactosidase was detected by adding X-Gal to the centre of the transformation plate (left panel, circle indicates the area of X-Gal application). The presence and function of the HYG3 marker was tested by transforming S. cerevisiae with pRS-AsTEF1p-ScRAD51-HYG3 and selecting the transformants in the presence of 100 µg/mL of hygromycin (right).