Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 5;7(7):192. doi: 10.3390/microorganisms7070192

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Comparison of two transformation methods in the Weihenstephan lager yeast strain WS 34/70. (A,C) A lithium-acetate/single strand DNA/polyethylene glycol (Li-Ac/ssDNA/PEG) and (B,D) a LiAc/PEG incubation combined with electroporation (Li-Ac/Ep) were used with two different antibiotic concentrations for the selection of transformants. The transformation of WS 34/70 with a freely replicative plasmid carrying a hygromycin resistance marker (pRS-AsTEF1p-ScRAD51-HYG3) yielded few transformants when using the Li-ac/ssDNA/PEG method (A,C). By contrast, the Li-Ac/Ep method together with antibiotic selection at 100 µg/mL final concentration (D), resulted in a higher transformation efficiency compared to the other combinations (A–C).