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. 2019 Jul 5;7(7):192. doi: 10.3390/microorganisms7070192

Figure 3.

Figure 3

PCR-based gene targeting in lager yeast. The Weihenstephan strain WS34/70 carrying either an empty vector (pHYG3; A) or a RAD51 plasmid (pRAD51, C) was transformed with disruption cassettes containing the YES1 marker harboring 50 bp of flanking homology regions for targeting to the ScHSP104 locus. We used a Li-Ac/Ep transformation protocol and selected transformants on YPD plates supplemented with hygromycin and G418 (100 µg/mL final concentration each). Restreaking of putative transformant colonies on new selective plates indicated stable transformants were only obtained in the lager yeast strain overexpressing pRAD51 (B,D).