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Diagnostic PCR for verification of correct marker integration. (A) Schematic presentation of PCR-based gene targeting of YES1 amplified with gene specific S1/S2-primers adding 50 bp of target homology region (orange bars) to ScHSP104 or ScADE2, respectively. Diagnostic G1/G2 and G3/G4 primers were used for verification of the correct insertion of YES1 and removal of the Sc HSP104 (B) and Sc ADE2 locus (C) ORFs.
