Table 3.
Primers used in this study.
| Primer Number | Primer Name | Sequence 5′→3′ * |
|---|---|---|
| 106 | 5′-AsTEF1p | GTCCAGAATAACATCAAATC |
| 107 | 3′-AsTEF1p | CTATAAAAAATGTTAGTATGGAG |
| 108 | 5′-AsTEFp-pRS | CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCTCGAGTCCAGAATAACATCAAATC |
| 109 | 3′-AsTEFp-lacZ | CAATCTTTGGATCGTTTAAATAAGTTTGAATTTTTTCAGTCATGTTCTATAAAAAATGTTAGTATGGAG |
| 226 | P3-pRS415-AsTEF1p | TGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGAAGCTTCGTACGCTGCAGGTCGGATCCCCCGGGGGCGCGCCGTCCAGAATAACATCAAATC |
| 227 | P4-AsTEF1p-kanR | GTTGGAGTTCAAACGTGGTCTGGAAACGTGAGTCTTTTCCTTACCCTATAAAAAATGTTAGTATGGAG |
| 228 | P5-kan-ORF | GGTAAGGAAAAGACTCACGTTTCCA |
| 229 | P6-kanR-pRS415 | GGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTTCTGATATCATCGATGAATTCGAGCTCGTTTAAACATTGGTAATAG |
| 230 | P7-5′-HYG3 | CTGACTTTTGTCTTGTTATGGACTCCATACTAACATTTTTTATAGAAAAAACCAGAATTGACTGCTACTTC |
| 231 | P8-3′-HYG3 | CTGATATCATCGATGAATTCGAGCTCGTTTAAACATTGGTAATAGGACCACCTTTGATTGTAAATAG |
| 253 | 5-HYG3+AD | GGCGCGCCAGATCTAGCCTCCTCAGAGAAAATTGCACAAAAAAAAGGAAGCTTCGTACGCTGCAGGTC |
| 254 | 3-HYG3+AD | TTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGACCACCTTTGATTGTAAATAG |
| 258 | 5-ScRAD51+AD | CTGACTTTTGTCTTGTTATGGACTCCATACTAACATTTTTTATAGTCTCAAGTTCAAGAACAACATATATCAG |
| 259 | 3-ScRAD51+AD | CTTTTTTTTGTGCAATTTTCTCTGAGGAGGCTAGATCTGGCGCGCCGAAAAATACATATATTTCATGGGTGACAG |
| 260 | 5-ScRAD51 | TCTCAAGTTCAAGAACAACATATATCAG |
| 261 | 3-ScRAD51 | GAAAAATACATATATTTCATGGGTGACAG |
| 344 | S1-LEU2 | GGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTAAAGTGCAATTCTTTTTCC |
| 345 | S2-LEU2 | CTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCGGTCGAGGAGAACTTC |
| 355 | G2-YES1 | GAATGAATCTACTGGTTTGG |
| 356 | G3-YES1 | GTGTCGGTATCGCAGAC |
| 369 | S1-ADE2 | CCTACTATAACATTCAAGAAAAACAAGAAAACCGGACAAAACAATCAAGTGTCCAGAATAACATCAAATC |
| 371 | S2-ADE2 | TATATCATTTTATATTATTTGCTGTGCAAGTATATCAATAAACTTATATATAATAAATTATTTTTATTGTTG |
| 373 | G1-ADE2 | GACTCTTGTTGCAGGGCT |
| 389 | G4-ADE2 | GTGATGCATTGAGCCGCC |
| 390 | S1-HSP104 | TATATTACTGATTCTTGTTCGAAAGTTTTTAAAAATCACACTATATTAAAGTCCAGAATAACATCAAATC |
| 391 | S2-HSP104 | AACAAAGAAAAAAGAAATCAACTACACGTACCATAAAATATACAGAATATTAATAAATTATTTTTATTGTTG |
| 392 | G1-HSP104 | CCCGTATTCTAATAATGGACC |
| 393 | G4-HSP104 | CAAACTTATGCAACCTGCCAG |
* Underlined sequences correspond to restriction sites.