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. 2019 Jul 20;9(7):148. doi: 10.3390/metabo9070148

Figure 4.

Figure 4

Eicosanoic, docosanoic and palmitic acid restricts in vitro USA300 growth in Nutrient Broth medium. (A) In vitro bacterial growth curves in Nutrient Broth and supplemented with DMSO (control), one fatty acid (30 µM) and gentamicin (100 µg/mL) were evaluated by measuring the absorbance at 2, 4, 6, 8 and 24 h. (B) In vitro bacterial growth at 6, 8 and 24 h in the presence of DMSO (C), docosanoic acid (D), eicosanoic acid (E), palmitic acid (P) or stearic acid (S). Data are expressed as means ± SE of three independent experiments performed in duplicates. One-way ANOVA and post hoc Tukey’s multiple comparison tests were performed to assess statistically significance across treatments. p-value ≤ 0.01 (**); ≤ 0.001 (***).