Figure 1.

A. Genetic origins of P_Rox promoters. Top panel: P_Rox132 is part of the upstream genetic region of ddcA (light grey). It starts with the RoxR recognition element (Rox RE), the regulatory regions (−35 and −10) and a ribosome binding site (RBS), which is located in the 5′ untranslated region (white). Bottom panel: P_Rox306 consists of the whole intergenic region between P. putida KT2440 genes PP_3332 and PP_3331.

B. DNA sequences of P_Rox132, P_Rox306 and P_Rox3061. The −35 and −10 regions predicted by BPROM (Solovyev and Salamov, 2011) are depicted in bold. Experimentally determined transcriptional start sites are marked with + 1. In P_Rox3061, the −10 region was mutated to the consensus sequence of the major σ‐factor of the σ⁷⁰ family, TATAAT. The initiation codon of the regulated gene is underlined. The sequence of the Rox RE is shaded, the putative RBS sequence depicted in italics.