Figure 2.

Expression of sfGFP by P. putida (A) under control of P_Rox132, P_Rox306 and P_Rox3061 and (B) under control of the constitutive promoter P_GAP and the inducible promoter P_BAD.

C. Comparison of sfGFP expression under control of P_Rox3061 in LB and MOPS medium.

D. Quantitative comparison of sfGFP accumulation within the cells with different promoters. P. putida cells containing plasmids for expression of sfGFP under control of the different promoters were cultivated in 500 ml shake flasks in LB or MOPS medium at 30°C, and OD ₅₇₈ and fluorescence intensity (FI, excitation: 485 nm, emission: 510 nm) were monitored. After reaching stationary phase, the cells were lysed, their proteins separated by SDS‐PAGE and sfGFP detected by means of anti‐sfGFP and secondary HRP‐conjugated antibodies. The detected band intensities were quantified and provided relative to the band intensity of P_Rox3061. The data are derived from one representative experiment, with the mean of three technical replicates shown. The error bars represent the standard deviation.