Figure 3.

A. Scheme of the unprocessed MATE‐mCherry fusion protein. After cleavage of the signal peptide (SP), the protein consists of a 6xHis epitope, the passenger mCherry, OmpT and fXa cleavage sites, a PEYFK epitope and the EhaA linker and β‐barrel (Sichwart et al., 2015).

B,C. Expression of MATE‐mCherry under control of P_Rox132, P_Rox306 and P_Rox3061. P. putida without plasmid and with plasmid pP_Rox132‐MATEmCherry (B), pP_Rox306‐MATE‐mCherry and pP_Rox3061‐MATE‐mCherry (C) were cultivated in LB medium in a 24‐well MTP at 30°C. OD ₅₇₈ and fluorescence intensity (FI, excitation: 580 nm, emission: 620 nm) were monitored. These data are mean values of biological triplicates. Error bars are not visible due to small standard deviations that are covered by the icons.