Figure 5.

RT‐qPCR analysis of MATE‐mCherry expression under control of P_BG35 (A), P_Rox132 (B), P_Rox306 (C) and P_Rox3061 (D). P. putida cells containing plasmids for expression of MATE‐mCherry under control of different promoters were cultivated in LB medium at 30°C, 200 rpm. At different time points, 2.5 × 10⁸ cells were removed from the cultures and the total amount of RNA was isolated. 1000 ng of total RNA was reversely transcribed to cDNA. 50 ng cDNA was amplified and analysed with specific primers in a qPCR cycler. The gene expression of MATE‐mCherry was determined relative to the gene expression of the reference gene rpoD (Fujita et al., 1995). The measured Cq values were analysed by applying a model of Pfaffl (2001). All RT‐qPCR analyses were performed as biological triplicates, each of them conducted as technical triplicates. Error bars indicate the standard deviation.