Figure 6.

Influence of conditioned medium (CM) on expression of MATE‐mCherry. P. putida pP_Rox306‐MATE‐mCherry was cultivated in LB medium to an OD ₅₇₈ = 0.8. Five millilitres of the cell suspension were harvested and suspended in mixtures of 4 ml fresh LB and 1 ml of different CM. The cells were then cultivated in a 24‐well MTP for another 12 h while monitoring OD ₅₇₈ and fluorescence intensity (FI).

A. 24 h CM, (B) 12 h CM, (C) 8 h CM, (D) 4 h CM. These data are mean values of biological triplicates. Error bars are not visible due to small standard deviations that are covered by the icons.