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Dual labelled probes for target and endogenous control genes. Minor groove binder-non-fluorescent quencher (MGB-NFQ) quencher was used in all probes.
| Gene | MGM-NFQ Dual Labelled Probe 5′–3′ | Fluorescent Dye |
|---|---|---|
| GAPDH | CCAGCCAAGTATGATGAT | VIC |
| GPX4 | CAGCCCAATGGAG | FAM |
| GSS | AGGAGGGAACAACCTG | FAM |
| GSR | CTGGCACTTCGGCTC | FAM |
