. 2019 Jun 28;8(7):201. doi: 10.3390/antiox8070201

Table 6.

Effect of sterigmatocystin and aflatoxin on relative gene expression of glutathione peroxidase 4 (GPX4), glutathione synthetase (GSS) and glutathione reductase (GSR) in the liver of broiler chickens (mean ± SD; n = 6, in a pool, equal amount of cDNA).

Gene Expression of GPX4
Samplings	Control	STC	PSTC	AFB₁
0 h	1.00 ± 0.05
Day 1	0.57 ^bc ± 0.02	0.63 ^c ± 0.03	0.52 ^ab ± 0.02	0.50 ^a ± 0.04
Day 2	0.57 ^a ± 0.03	0.59 ^ab ± 0.01	0.62 ^b ± 0.03	0.63 ^b ± 0.02
Day 3	0.67 ^a ± 0.03	0.62 ^a ± 0.04	0.71 ^a ± 0.07	0.60 ^a ± 0.02
Day 7	0.51 ^ab ± 0.04	0.48 ^a ± 0.03	0.67 ^c ± 0.06	0.59 ^b ± 0.04
Day 14	0.48 ^a ± 0.03	0.57 ^c ± 0.05	0.50 ^ab ± 0.03	0.55 ^bc ± 0.03
Gene Expression of GSS
Samplings	Control	STC	PSTC	AFB₁
0 h	1.00 ± 0.02
Day 1	0.55 ^d ± 0.04	0.46 ^c ± 0.02	0.38 ^ab ± 0.05	0.34 ^a ± 0.05
Day 2	0.45 ^a ± 0.05	0.57 ^b ± 0.03	0.52 ^ab ± 0.05	0.54 ^a ± 0.06
Day 3	0.66 ^ab ± 0.05	0.61 ^ab ± 0.08	0.60 ^a ± 0.08	0.70 ^b ± 0.05
Day 7	0.41 ^a ± 0.04	0.50 ^b ± 0.05	0.70 ^c ± 0.06	1.04 ^d ± 0.02
Day 14	1.03 ^c ± 0.06	0.93 ^b ± 0.05	0.74 ^a ± 0.09	0.91 ^b ± 0.04
Gene Expression of GSR
Samplings	Control	STC	PSTC	AFB₁
0 h	1.00 ± 0.03
Day 1	0.43 ^b ± 0.06	0.41 ^b ± 0.05	0.28 ^a ± 0.03	0.24 ^a ± 0.02
Day 2	0.36 ^a ± 0.05	0.40 ^ab ± 0.06	0.41 ^ab ± 0.05	0.46 ^b ± 0.04
Day 3	0.47 ^a ± 0.04	0.50 ^a ± 0.07	0.68 ^b ± 0.10	0.43 ^a ± 0.03
Day 7	0.42 ^a ± 0.03	0.39 ^a ± 0.07	0.55 ^b ± 0.07	0.84 ^c ± 0.05
Day 14	0.40 ^a ± 0.04	0.64 ^b ± 0.08	0.41 ^a ± 0.06	0.44 ^a ± 0.08

^a,b Different superscripts in the same rows mean significant difference at p < 0.05 level. STC—1590 µg STC/kg feed; PSTC—1570.5 µg STC/kg feed; AFB₁—149.1 µg AFB₁/kg feed.