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Purification summary of histidine-rich protein 2 (HRP-II). Ni–NTA—nickel–nitrilotriacetic acid.
| Purification Step | Total Protein (μg) | Total Activity (U) | Specific Activity (U/mg) | Yield (%) | Purification (folds) |
|---|---|---|---|---|---|
| Crude broth | 17.8 | 0.40 | 22.5 | 100.0 | 1.0 |
| Ni–NTA affinity column | 5.5 | 0.32 | 57.0 | 79.1 | 2.5 |
