Figure 1.

Cyclopentylidene-[4-(4-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2) inhibits C. albicans growth. (a) Spot dilution assays of C. albicans wt and hat1Δ/Δ strains using plates containing the indicated histone acetyltransferase (HAT) inhibitors in the absence or presence of methyl methanesulfonate (MMS). Cells were incubated for 3 days prior to imaging. Concentrations used were 200 µM for CPTH2, anacardic acid, and MB-3, 100 µM for garcinol, and 0.03% for MMS. (b,c) Spot dilution assays as described above (a), with 50 µM and 25 µM CPTH2, respectively. (d) Liquid growth inhibition assay in YPD medium. C. albicans wt cells were incubated with the indicated concentrations of CPTH2 for 24 h prior to OD₆₀₀ measurement. (e) Liquid growth inhibition assay as described above (d), using RPMI 1640 medium. Cells were incubated for 24 h prior to OD₆₀₀ measurement. (f) Growth curves of C. albicans wt cultures in the absence or presence of CPTH2. (g) Survival of cells upon CPTH2 treatment. Cells were grown in YPD medium and treated with 10 µM CPTH2 for the indicated time. CFUs were determined by plating and colony counting. (d–g) Data shown are mean ± sd of three (e–g) or four (d) biological replicates.