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Levels of factors used in Box-Behnken design (BBD).
| Factors | Range and Level | ||
|---|---|---|---|
| −1 | 0 | 1 | |
| X1 (the amount of egg phosphatidylcholine (PC)) | 10 mg | 25 mg | 40 mg |
| X2 (the mass ratio of egg PC-to-cholesterol (Chol)) | 1:1 | 3:1 | 5:1 |
| X3 (the mass ratio of lipid-to-drug) | 1:2 | 2:1 | 3.5:1 |
