Figure 2.

Adolescent alcohol drinking decreases myelinated fiber density in male, but not female, rats. (a) Adolescent alcohol drinking decreases myelinated fiber density in the Cg1 of males (* p = 0.01), with no measurable changes detected in females. Blue circular markers indicate the individuals used for representative images in panel b. Note: the fiber density means ± SEM were previously published for males [18]. (b) Representative microscopic images from each treatment group, with fibers displayed in red to show density threshold measurements. These representative images were taken with a 5× objective on a light microscope and processed in Image J (open source from https://imagej.net/ImageJ) for enhanced visualization for this figure. Myelin density data were analyzed on scanned images using Aperio ImageScope software (Leica Biosystems Imaging Inc., Vista, CA, USA), as described in the methods.