Table 7.

List of ruminal fluid metabolites that showed significant difference between CON ¹ and TR ² groups ³.

Compounds	RT 4	Mass	VIP 5	FDR 6	P-Value	FC 7
Amino acids
Glutamine	13.75	125	1.69	0.035	0.010	1.85
Isoleucine	10.55	158	1.535	0.043	0.026	2.148
Ornithine	16.91	174	1.558	0.046	0.041	1.948
Oxyproline	13.61	156	1.507	0.044	0.030	3.209
Alanine	7.86	116	1.672	0.046	0.042	4.227
Serine	11.44	204	1.441	0.039	0.016	3.133
Fatty acids
Malonate	13.24	81	1.776	0.010	<0.001	5.93
Benzoic acid	10.15	179	1.519	0.043	0.028	0.557
Pimelic acid	14.58	125	2.125	0.010	<0.001	2.903
Glutaconic acid	12.6	55	1.255	0.045	0.034	0.345
3-Hydroxyphenylacetic acid	14.65	164	1.788	0.043	0.020	0.44
Azelaic acid	16.72	317	1.532	0.043	0.028	0.477
Lipids
Methyl Palmitoleate	17.98	97	1.498	0.046	0.044	0.607
Methyl Phosphate	9.14	241	1.54	0.034	0.007	12.668
Sugars
6-Deoxy-d-glucose	15.74	117	1.838	0.015	0.002	3.49
Mannose	17.53	160	1.089	0.046	0.046	0.588
d-Talose	17.84	299	1.268	0.045	0.034	4.233
Organic acids
d-galacturonic acid	18.11	333	1.408	0.046	0.046	3.234
Salicylic acid	13.46	267	2.165	0.010	<0.001	0.063
Succinic acid	10.88	147	1.457	0.034	0.007	2.066
Amines
N-Acetyl-beta-d-mannosamine	19.4	73	1.464	0.043	0.026	1.809
Malonamide	13.93	174	1.401	0.043	0.022	2.034
5-Methoxytryptamine	22.8	174	1.714	0.045	0.036	2.049
Indole-3-acetamide	18.53	318	1.863	0.035	0.011	19.487
Nucleosides
Thymine	12.04	255	1.923	0.046	0.039	3.574
2′-Deoxyadenosine	24.07	192	1.446	0.043	0.025	3.61
Other metabolites
Taxifolin	26.33	179	1.804	0.035	0.011	31.784
Resorcinol	11.82	239	1.614	0.039	0.016	2.505
Alizarin	23.92	192	2.018	0.038	0.013	2.407

¹ CON (control group) = dairy cows fed total mixed ration (TMR). ² TR (treatment group) = dairy cows fed pelleted high-starch diet. ³ A metabolite is assigned as significantly different if its VIP is > 1 with and FDR < 0.05. ⁴ RT = retention time. ⁵ VIP = variable importance in projection. ⁶ FDR = false discovery rate. ⁷ FC = fold change, the ratio of mean value of peak area obtained from the cows in CON and TR groups. If FC > 1.5, means that this metabolite is higher in TR group than in CON group. If FC < 0.67, means that this metabolite is lower in TR group than in CON group.