DRMs compression isotherms. (a) Compression isotherms of Langmuir films prepared with DRMs of WT (red) and Cav-1 KO (black) mice and isotherms relative to the same samples after protein removal (green and blue). (b) Protein-free DRMs isotherms (WT pink and KO blue) in comparison to the isotherm of a simplified “raft” model made of cholesterol, monosialotetrahexosylganglioside (GM1), and dipalmitoylphosphatidylcholine (DPPC) (red) and to the single lipid components (black). (c) The reconstitution of Cav-1 in proteoliposomes: Isotherm obtained after reconstitution (red) in comparison with the KO-DRMs isotherm (black) and the protein-free sample obtained from WT-DRMs, used to prepare proteoliposomes (green). Lo, liquid-ordered phase; Ld, liquid-disordered phase; GM1, monosialotetrahexosylganglioside; DPPC, dipalmitoylphosphatidylcholine.