Figure 1.

Inhibited cell proliferation and induces G₂/M cell cycle arrest in osteosarcoma MG-63 cells. (A) Chemical structure of diallyl disulfide (DADs). (B) Cell viability. MG-63 cells were treated with the indicated dose of DADs (0, 20, 40, 60, 80, and 100 μM) for different times (24, 48, and 72 h). Cell viability was detected by CCK8 assay (n = 3). (C,D) Clone formation. MG-63 cells were treated with 0, 20, 60, and 100 μM DADs, and the number of cell colonies was measured by clone formation assay 9 days later. (E,F) Cells were treated with DADs for 24 h, and the cell cycle was detected by flow cytometry. G₂/M cell cycle arrest was observed in MG-63 cells. The percentage of the sub G₁, G₀/G₁, S, and G₂/M phase cell populations were represented by the mean ± SD of at least three independent experiments. Statistical differences were analyzed by student’s t-test (* p < 0.05, ** p < 0.01 compared with control group).