Figure 1.

APR-246 inhibits ALDH activity in TNBC cells.

Notes: MDA-MB-231 (A) or MDA-MB-435 (B) TNBC cells were treated in petri dishes with APR-246 or PBS vehicle (control) DMEM/F12 with 5% FBS for 24 hrs, then incubated with ALDEFLUOR substrate ±5 mM DEAB (an inhibitor of ALDH) as described by the ALDEFLUOR assay kit’s manufacturer (STEMCELL Technologies). Cells (2.5×10⁴ cells/sample) were subsequently analyzed using flow cytometry. The ALDH gate was set using the negative control (+ DEAB). Viable cells were gated using propidium iodide. Results represent mean±SEM (n=3). *Significantly different from control (P<0.05, one-way ANOVA) followed by a Student–Newman–Keuls multiple comparison test.

Abbreviations: ALDH, aldehyde dehydrogenase isoform 1; ANOVA, analysis of variance; DEAB, N,N-diethylaminobenzaldehyde; PBS, phosphate-buffered saline SEM, standard error of the mean; TNBC, triple-negative breast cancer.