Figure 2.
APR-246 inhibits mammosphere formation in TNBC cells.
Notes: MDA-MB-231 and MDA-MB-435 cells were seeded into ultra-low adherent 6-well plates (5×103 cells/well) and treated for up to 6 days with 1–10 µM APR-246 or medium (control) in Complete Mammocult medium. Cells were retreated with fresh APR-246 or Complete Mammocult medium every 48 hrs. Representative light microscopic images of mammospheres formed on day 4 are shown in (A) and bar graph representing inhibition of mammospheres is shown in (B). Bar represents 100 μm. Inserts show an enlarged image of mammosphere. *Significantly different from control (P<0.05, one-way ANOVA) followed by a Student–Newman–Keuls multiple comparison test. **Significantly different from control and 1 μm APR-246 groups (P<0.05, one-way ANOVA) followed by a Student–Newman–Keuls multiple comparison test.
Abbreviation: TNBC, triple-negative breast cancer.