Figure 1.

Arabidopsis WRINKLED1 (AtWRI1) structural features and regulatory mechanism of AtWRI1-mediated oil biosynthesis. (A) Schematic diagram of domains and motifs of AtWRI1, including two APETALA2 (AP2) domains, three intrinsically disordered regions (IDRs), a functional motif of “VYL”, the transactivation domain (TAD), the binding motifs of 14-3-3 and E3 ligase adaptor (BPM), PEST degradation signal sequence, and the KIN10 phosphorylation sites. (B) Post-translational regulatory mechanisms for AtWRI1 mediated by phosphorylation and newly identified interacting regulators, including Mediator Subunit 15 (MED15), BPMs, and kinase KIN10. The regulators modulate the protein stability and transcriptional activity of AtWRI1 via interactions and modifications, which in turn alters the gene expression of AtWRI1 targets.