Figure 3.
The role of tRFGluTTC in proliferation and differentiation of preadipocytes. (A) The proliferation ability of 3T3-L1 preadipocyte was measured by CCK-8. (B,C) 3T3-L1 preadipocyte proliferation was measured by EdU staining. (D) The relative mRNA expression levels of cell cycle regulatory factors (CDK4, Cyclin E, and Cyclin D1) by RT-PCR. (E,F) Oil Red O staining and triglyceride content of terminally differentiated (Day 8) adipocytes that transfected with tRFGluTTC mimics and inhibitors. (G) The relative expression of KLF family (KLF9, KLF11, KLF12, and KLF13) by RT-PCR. (H) The relative expression of adipogenic transcription factors (aP2, peroxisome proliferator-activated receptor gamma (PPARγ), and CCAAT enhancer binding protein alpha (C/EBPα)) by RT-PCR. (I) The relative expression of genes related to fatty acid synthesis and oxidation by RT-PCR. All data were presented as means ± standard deviation. * p < 0.05.