Ordination plot of study regions (five in total) across a biogeographic gradient based on principal component analysis of interior Douglas-fir first-order root traits. C, root carbon concentration (%); N, root nitrogen concentration (%); SRA, specific root area (cm2g−1); SRL, specific root length (mg−1); RTD, root tissue density (mgcm−3); BrIntensity, branching intensity (the number of first- order root/length of second- order root; cm−1); DBI, dichotomous branching index, values closer to 0 indicate a dichotomous branching pattern and values closer to 1, a herringbone branching pattern, see [31]. PC, principal component, PC1 = 30%; PC2 = 22%, see Figure S1.