Figure 5.

Production of indoline‐betacyanin in 2L bioreactor. Different trends in the synthesis of indoline‐betacyanin can be observed when pH is controlled to 7.0 (A) and when pH drops freely to 5.8 (B). Pictures are shown after 96‐h incubation. When carrying out the reaction at pH 7.0 (C), the consumption of alkali increases progressively due to the tendency of acidification in the synthesis of betalamic acid. D: Effect of stopping the consumption of alkali after the addition of reactives on pH when it evolves freely in the reactor. *Medium change: cells in LB medium were centrifuged and the pellet was resuspended in water.