Table 1.
The surgical materials for different kinds of blood sample collection approaches.
| Bleeding Site | Materials | Appliance | Animal State | Bleeding Rate | Blood Volume |
|---|---|---|---|---|---|
| Saphenous vein | Needles (23G), Vaseline | Electric shaver | Awake | 3.66 ± 0.72 μL/s | ~400 μL |
| Tail vein | Needles (23G), Syringe (1 mL), PE50 tubing |
Rat holder, Tweezer |
Awake | 8.50 ± 1.70 μL/s | ~400 μL |
| Tail vein | Needles (23G), Syringe (1 mL), PE50 tubing (0.058 cm × 0.097 cm) |
Tweezer | Anesthetic (sevoflurane/pentobarbital sodium) | 8.50 ± 1.70 μL/s | ~500 μL |
| Inferior thoracic vena cava | Syringe (2 mL) | Scissor | Anesthetic (pentobarbital sodium) | ~2 mL, even more |