KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-IFI16 (1G7) | Santa Cruz | Cat# sc-8023 RRID:AB_627775 |
| Mouse monoclonal anti-HA tag (HA.C5) | Abcam | Cat# ab18181 RRID:AB_444303 |
| Rabbit monoclonal anti-HA tag (C29F4) | Cell Signaling | Cat# 3724S RRID:AB_1549585 |
| Rabbit monoclonal anti-STING (D2P2F) | Cell Signaling | Cat# 13647 RRID:AB_2732796 |
| Mouse monoclonal anti-HIV-1 Env (16H3) | NIH AIDS Reagent Program | Cat# 12559 |
| Mouse monoclonal anti-HIV-1 p24 (39/5.4A) | Abcam | Cat# ab9071 RRID:AB_306981 |
| Rabbit polyclonal anti-Vpu | S. Bolduan | N/A |
| Rabbit polyclonal anti-AU1 tag | Novus Biologicals | Cat# NB600-453 RRID:AB_10003439 |
| Mouse monoclonal anti-β actin | Abcam | Cat# ab8226 RRID:AB_306371 |
| Rabbit polyclonal anti-GAPDH | Biolegend | Cat# 631401 RRID:AB_2247301 |
| Mouse monoclonal anti-GAPDH | Santa Cruz | Cat# sc-365062 RRID:AB_10847862 |
| Rabbit polyclonal anti-GFP | Abcam | Cat# ab290 RRID:AB_303395 |
| Goat polyclonal anti-Sp1 | Abcam | Cat# ab157123 |
| Rabbit polyclonal anti-Sp1 | Abcam | Cat# ab227383 |
| IRDye® 680RD Goat anti-Mouse IgG (H + L) | LI-COR | Cat# 926-68070 RRID:AB_10956588 |
| IRDye® 680RD Goat anti-Rabbit IgG (H + L) | LI-COR | Cat# 926-68071 RRID:AB_10956166 |
| IRDye® 680RD Donkey anti-Goat IgG (H + L) | LI-COR | Cat# 926-68074 RRID:AB_10956736 |
| IRDye® 800CW Goat anti-Mouse IgG (H + L) | LI-COR | Cat# 926-32210 RRID:AB_621842 |
| IRDye® 800CW Goat anti-Rabbit IgG (H + L) | LI-COR | Cat# 926-32211 RRID:AB_621843 |
| Donkey polyclonal anti-mouse, Alexa Fluor 647 | ThermoFisher | Cat# A-31571 RRID:AB_162542 |
| Goat polyclonal anti-mouse, PE | ThermoFisher | Cat# P-852 RRID:AB_2539848 |
| Anti-HIV-1 p24 core antigen (MAK183) | ExBIO | Cat# 11-CM006-BULK |
| Rabbit anti-p24 antiserum obtained by immunization of rabbits | Eurogentec | N/A |
| Peroxidase-AffiniPure goat anti-rabbit IgG, Fc fragment specific antibody | Dianova | Cat#
111-035-008 RRID:AB_2337937 |
| Rabbit polyclonal anti-human IL-4 | PeproTech | Cat# 500-P24 RRID:AB_147568 |
| Goat polyclonal anti-human IL-12 | PeproTech | Cat# 500-P154G RRID:AB_147809 |
| Mouse monoclonal anti-CD4, APC conjugated | Thermo Fisher | Cat# MHCD0405 RRID:AB_10373698 |
| Mouse monoclonal anti-HIV-1 core, FITC conjugated | Beckman Coulter | Cat# 6604665 |
| Bacterial and Virus Strains | ||
| Escherichia coli XL-2 blue™ | Stratagene | Cat# 200150 |
| XL2-Blue MRF’ TM Ultracompetent cells | Agilent Technologies | Cat# 200151 |
| Friend virus | U. Dittmer (University of Essen) | N/A |
| Sendai virus | G. Kochs (University of Freiburg) | N/A |
| Biological Samples | ||
| Human: Peripheral blood mononuclear cells | DRK-Blutspendedienst Baden-Württemberg-Hessen, Ulm, Germany | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| L-Glutamine | Pan Biotech | Cat# P04-80100 |
| Penicillin-Streptomycin | ThermoFisher | Cat# 15140122 |
| HAT supplement | ThermoFisher | Cat# 21060017 |
| Blasticidin | InvivoGen | Cat# ant-bl-1 |
| Zeocin | InvivoGen | Cat# ant-zn-1 |
| Normocin | InvivoGen | Cat# ant-nr-1 |
| QUANTI-Blue | InvivoGen | Cat# rep-qb2 |
| Human IL-2 IS, premium grade | MACS Miltenyi Biotec | Cat# 130-097-745 |
| Recombinant human IL-2 | NIH AIDS Reagent | Cat# 136 |
| Remel™ PHA purified | ThermoFisher | Cat# R30852801 |
| Biocoll separating solution | Biochrom | Cat# L6115 |
| Lymphoprep | Stemcell | Cat# 07851 |
| Human Interferon α 2a | pbl assay science | Cat# 11101-1 |
| Human Interferon γ | Sigma-Aldrich | Cat# I3265 |
| Dynabeads Human T-Activator CD3/CD28 | ThermoFisher | Cat# 111.32D |
| Tumor Necrosis Factor-α human | Sigma-Aldrich | Cat# H8916-10UG |
| Human M-CSF | R&D Systems | Cat# 216-MC-01M |
| Phorbol 12-myristate 13-acetate (PMA) | Sigma-Aldrich | Cat# 79346 |
| Recombinant human TGF-β1 | PeproTech | Cat# 100-21 |
| cGAMP | BioLog | Cat# C161 |
| HT-DNA | Sigma Aldrich | Cat# D6898 |
| Lipofectamine RNAiMAX Transfection Reagent | ThermoFisher | Cat# 13778150 |
| TransIT®-LT1 Transfection Reagent | Mirus | Cat# MIR 2305 |
| β-mercaptoethanol | Sigma Aldrich | Cat# M6250-100ML |
| HIV-1 p24 protein (ELISA standard) | Abcam | Cat# 43037 |
| KPL SureBlue™ TMB Microwell Peroxidase Substrate | Medac | Cat# 52-00-04 |
| Sulfuric acid concentrate for 1l standard solution 0.5 M H2SO4 | Sigma-Aldrich | Cat# 38294-1EA |
| 4X Protein Sample Loading Buffer | LI-COR | Cat# 928-40004 |
| 2-Mercaptoethanol | Sigma-Aldrich | Cat# M6250-100ML |
| NucRed Live 647 ReadyProbes Reagent | ThermoFisher | Cat# R37106 |
| Fixable Viability Dye eFluor™ 450 | eBioscience | Cat# 65-0863-14 |
| Benzonase Nuclease | Sigma-Aldrich | Cat# E1014-25KU |
| Mithramycin A | Cayman Chemicals | Cat# 11434 |
| MTT (Thiazolyl Blue Tetrazolium Bromide) | Sigma-Aldrich | Cat# M5655 |
| Critical Commercial Assays | ||
| RosetteSep™ Human CD4+ T Cell Enrichment Cocktail | Stem Cell Technologies | Cat# 15062 |
| EasySep™ Human Naïve CD4+ T Cell Isolation Kit | Stemcell | Cat# 19555 |
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent | Cat# 200522 |
| Phusion High-Fidelity PCR Kit | ThermoFisher | Cat# F553L |
| DNA Ligation Kit Ver. 2.1 | TaKaRa | Cat# 6022 |
| GalScreen | Applied Bioscience | Cat# T1027 |
| RNeasy Plus Mini Kit | QIAGEN | Cat# 74136 |
| DNA-free™ DNA Removal Kit | ThermoFisher | Cat# AM1906 |
| PrimeScript RT Reagent Kit | TAKARA | Cat# RR037A |
| TaqMan™ Fast Universal PCR Master Mix | ThermoFisher | Cat# 4352042 |
| GAPDH Endogenous Control (VIC/TAMRA) | ThermoFisher | Cat# 4310884E |
| Luciferase Cell Culture Lysis 5X Reagent | Promega | Cat# E1531 |
| Luciferase Assay System 10-pack | Promega | Cat# E1501 |
| Coelenterazine (native-CTZ) | p.j.k. GmbH | Cat# 102173 |
| FIX & PERM Kit (CE-IVD) | Nordic-MUbio | Cat# GAS-002-1 |
| Pierce Protein A/G Magnetic Beads | ThermoFisher | Cat# 88802 |
| Pierce Gold BCA Protein Assay Kit | ThermoFisher | Cat# A53225 |
| TransAM Sp1 Transcription Factor Assay Kit | ActiveMotif | Cat# 41296 |
| C-Type RT Activity Kit | CAVIDI | Cat# 51020 |
| Nano-Glo Luciferase Assay System | Promega | Cat# N1110 |
| BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution | Becton Dickinson | Cat# 554722 |
| BD Perm/Wash™ Perm/Wash Buffer | Becton Dickinson | Cat# 554723 |
| Experimental Models: Cell Lines | ||
| Human: HEK293T cells | ATCC | Cat# CRL-3216 RRID: CVCL_0063 |
| Human: TZM-b1 cells | NIH AIDS Reagent Program | Cat# 8129 RRID: CVCL_B478 |
| Human: HL116 cells | Christine Goffinet | N/A |
| Human: HEK-Blue | InvivoGen | Cat# hkb-ifnab |
| Human: HAP1 cells | Horizon | Cat#
HZGHC001141c002 RRID:CVCL_TQ04 |
| Human: Sp1 KO HAP1 cells | Horizon | Cat#
HZGHC001141c002 RRID:CVCL_TQ04 |
| Human: THP-1 cells | ATCC | Cat#TIB-202 |
| Human: J-Lat cells (clone 10.6) | NIH AIDS Reagent Program | Cat# 9849 RRID:CVCL_8281 |
| Experimental Models: Organisms/Strains | ||
| B6(129S4)-Del(1Aim2-Ifi205)1Stsn/J Mice | The Jackson Laboratory | Stock No: 029472 |ALR− |
| Oligonucleotides | ||
| Primers used for generation of pCG-based expression constructs | see Table S1 of this paper | N/A |
| Primers used for generation of HIV-1 LTR F-luc reporter constructs | see Table S2 of this paper | N/A |
| Primers used for generation of chimeric HIV-1 proviral constructs | see Table S3 of this paper | N/A |
| Primers and fluorescent probes for qRT-PCR of viral transcripts | see Table S4 of this paper | N/A |
| siRNA targeting IFI16 | Invitrogen | Cat# IFI16HSS105205/IFI16HSS105206/IFI16HSS105207 |
| Non-targeting control siRNA: UUCUCCGAACGUGUCACGUdTdT | Eurofins MWG | N/A |
| Recombinant DNA | ||
| Plasmid: pLKO.1 puro | Stewart et al., 2003 | Addgene Cat# 8453 |
| Plasmid: pCG_IFI16 | This paper | N/A |
| Plasmid: pCG_IFI16 (SV40 NLS) | This paper | N/A |
| Plasmid: pCG_IFI16 (HIV-1 Rev NES) | This paper | N/A |
| Plasmid: pCG_IFI16 NLS mut (R96A, K97A, R98A, K99A, K100A, K128A, R129A, K130A, K131A, K134A, K136A, K140A) | This paper | N/A |
| Plasmid: pCG_IFI16 (K99A, K128A) | This paper | N/A |
| Plasmid: pCG_IFI16 ΔNLS1/2 (ΔR96-100K/K128-131K) | This paper | N/A |
| Plasmid: pCG_IFI16 IRES BFP | This paper | N/A |
| Plasmid: pCG_STING IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_cpz IFI16 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_agm IFI16 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_mac IFI16 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 Pyrin mut (S27A, L28A, D50A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔNLS1/2 (ΔR96-100K/K128-131K) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔPyrin (Δ1-87) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔPyrin-linker (Δ1-190) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔHinA (Δ191-393) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔHinA-linker (Δ191-509) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 HinB mut (K572A, K607A, R611A, S614A, K618A, N654A, K676A, K678A, K703A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔHin HinB mut C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔHinA-linker HinB mut C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 ΔHinB (Δ509-729) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 Pyrin-linker (Δ191-729) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 Pyrin-only (Δ88-729) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (R427A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (E463A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (G464A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (H466A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (G469A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (M472A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (V615A) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (S409R, N413Y) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (S409R) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_IFI16 (N413Y) C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_AIM2 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_mouse p204 C-HA IRES BFP | This paper | N/A |
| Plasmid: pCG_STING IRES BFP | This paper | N/A |
| Plasmid: pCG_Sp1 C-HA IRES BFP | This paper | N/A |
| Plasmid: pBR322_NL4-3 IRES eGFP | Frank Kirchhoff (Ulm University) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype A 191845 (transmitted founder virus) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype A 9004SDM (transmitted founder virus) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype A R880F (transmitted founder virus) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pBR322_HIV-1 M subtype A 191084 (transmitted founder virus) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype B CH058.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pCR-XL-TOPO HIV-1 M subtype B CH040.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pCR-XL-TOPO HIV-1 M subtype B CH077.t (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pBR332_HIV-1 M subtype B CH470 (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO HIV-1 M subtype B pWITO.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype B CH106.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pCR-XL-TOPO HIV-1 M subtype B pTHRO.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pHIV-1 AD8 | V. Planelles (Zimmerman et al., 2006) | N/A |
| Plasmid: pBR322_HIV-1 M subtype B REJO.c (transmitted founder virus) | B. H. Hahn (Ochsenbauer et al., 2012) | N/A |
| Plasmid: pCR-XL TOPO_HIV-1 M subtype C CH042 (transmitted founder) | B. H. Hahn (Freel et al., 2012) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype C CH162 (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pCR-XL TOPO_HIV-1 M subtype C CH185 (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype C CH198 (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pUC57 HIV-1 M subtype C CH167 (chronic virus) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pBR332_HIV-1 M subtype C CH850 (transmitted founder) | B. H. Hahn (Fenton-May et al., 2013) | N/A |
| Plasmid: pUC57rev_HIV-1 M subtype C CH293 (chronic virus) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype C CH164 (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype C ZM247Fv-1 (transmitted founder virus) | B. H. Hahn (Salazar-Gonzalez et al., 2009) | N/A |
| Plasmid: pBR332_HIV-1 M subtype C CH236 (transmitted founder) | B. H. Hahn (Fenton-May et al., 2013) | N/A |
| Plasmid: pUC57_HIV-1 M subtype C CH200a (transmitted founder) | B. H. Hahn (Parrish et al., 2013) | N/A |
| Plasmid: pBR322_HIV-1 M subtype C CH264 (transmitted founder) | B. H. Hahn (Salazar-Gonzalez et al., 2009) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype D 191647 (transmitted founder) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype D 191859 (transmitted founder) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype D 191882 (transmitted founder) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M subtype D 190049 (transmitted founder) | B. H. Hahn (Baalwa et al., 2013) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M CH058 with 3’ and 5’ LTR of THRO | This paper | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M CH040 with 3’ and 5’ LTR of THRO | This paper | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 M THRO with 3’ and 5’ LTR of CH058 | This paper | N/A |
| Plasmid: pCR-XL-TOPO SIVcpz Ptt EK505 | B. H. Hahn (Keele et al., 2006) | N/A |
| Plasmid: pCR-XL-TOPO SIVcpz Ptt MB897.2 | F. Kirchhoff (Bibollet-Ruche et al., 2012) | N/A |
| Plasmid: pBluescript II KS+_HIV-2 AB 7312A | NIH AIDS Reagent Program | Cat# 3551 |
| Plasmid: pBR322_HIV-2 ROD9 eGFP | J. Münch(Ulm University) | N/A |
| Plasmid: pBR322_HIV-2 ROD10 | J. Münch (Ulm University) | N/A |
| Plasmid: pSP65 HIV-2 A ST | NIH AIDS Reagent Program (Kumar et al., 1990) | Cat# 12444 |
| Plasmid: pHIV-2 A KR | B. H. Hahn (Talbott et al., 1993) | N/A |
| Plasmid: pGEM_SIVsmm pg | F. Kirchhoff (Ulm University) | N/A |
| Plasmid: pBR322hl_SIVsmm L1.RM174.V1.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pBR322hl_SIVsmm L1.RM174.V2.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pBR322hl_SIVsmm L2.RM136.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pBR322hl_SIVsmm L3.RM175.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pBR322hl_SIVsmm L4.RM57.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pBR322MCS_SIVsmm Outlier.DT17.tf | B. H. Hahn (Mason et al., 2016) | N/A |
| Plasmid: pCR-XL-TOPO_HIV-1 N DJO0131 | F. Bibollet-Ruche (Bodelle et al., 2004) | N/A |
| Plasmid: pBluescript_HIV-1 O pCMO2.5 | H.G. Kräusslich (Tebit et al., 2004) | N/A |
| Plasmid: pSMART-topo_HIV-1 O RBF206 | F. Bibollet-Ruche (Mack et al., 2017) | N/A |
| Plasmid: pBR322_HIV-1 O BCF183 | B. Hahn | N/A |
| Plasmid: pBR322_HIV-1 P RBF168 | B. H. Hahn (Plantier et al., 2009) | N/A |
| Plasmid: pBR322_SIVmac 239 | F. Kirchhoff (Ulm University) | N/A |
| Plasmid: pCR-XL-TOPO_SIVagm Sab92018ivTF | F. Kirchhoff (Gnanadurai et al., 2010) | N/A |
| Plasmid: pGL3-enhancer HIV-1 M NL4-3 LTR (wild type) firefly luciferase reporter | This paper | N/A |
| Plasmid: pGL3-enhancer HIV-1 M NL4-3 LTR (Sp1 site mutated) firefly luciferase reporter | This paper | N/A |
| Plasmid: pGL3-enhancer_HIV-1 M NL4-3 LTR (NF-κB/NFAT site mutated) firefly luciferase reporter | This paper | N/A |
| Plasmid: pGL3-enhancer_HIV-1 M NL4-3 LTR (USF site mutated) firefly luciferase reporter | This paper | N/A |
| Plasmid: pGL3-enhancer_HIV-1 M NL4-3 LTR (TCF-1α site mutated) firefly luciferase reporter | This paper | N/A |
| Plasmid: pGL3-enhancer_HIV-1 M NL4-3 LTR (NF-IL6 site mutated) firefly luciferase reporter | This paper | N/A |
| Plasmid: pCG_NL4-3 Tat IRES eGFP | F. Kirchhoff (Ulm University) | N/A |
| Plasmid: pTK-Red_HSV-TK firefly luciferase reporter | ThermoFisher | Cat# 16157 |
| Plasmid: pCMV-Red_CMV-IE firefly luciferase reporter | ThermoFisher | Cat# 16156 |
| Plasmid: pTAL_Gaussia luciferase reporter | D. Sauter (Hotter et al., 2013) | N/A |
| Plasmid: p_HIV-1 M LAI1 wild type | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-2d15tetO (ΔTAR loop) (tat stop) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-2d15tetO (ΔTAR loop) (Tat Y26A) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-tetOcmv (tat stop) (lacks all HIV-1 U3 and TAR sequences) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-tetOcmv (lacks all HIV-1 U3 and TAR sequences) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-tetOcmv (lacks all HIV-1 U3 except for Sp1 binding sites; lacks TAR sequences) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-1 M LAI1 rtTA-tetOcmv (tat stop) (lacks all HIV-1 U3 except for Sp1 binding sites; lacks TAR sequences) | A.T. Das (Das et al., 2011) | N/A |
| Plasmid: p_HIV-LTR-2d15tetO-dNF-luc | A.T. Das (Turrini et al., 2015) | N/A |
| Plasmid: p_HIV-LTR-2d15tetO-dNF-mSp1-luc (deletion of 1 Sp1 site) | A.T. Das (Turrini et al., 2015) | N/A |
| Plasmid: p_HIV-LTR-2d15tetO-dNF-mSp2-luc (deletion of 2 Sp1 sites) | A.T. Das (Turrini et al., 2015) | N/A |
| Plasmid: p_HIV-LTR-2d15tetO-dNF-mSP-luc (mutation of all 3 Sp1 sites) | A.T. Das (Turrini et al., 2015) | N/A |
| Plasmid: p_HIV-LTR-2d15tetO-X/H-luc | A.T. Das (Turrini et al., 2015) | N/A |
| Plasmid: pHIV-1 NL4-3-deltaEnv-nLuc-2ANef | This paper (generated by Laura Martins in Planelles lab) | N/A |
| Plasmid: pCMV-VSV-G | Stewart et al., 2003 | Addgene Cat# 8454 |
| Plasmid: p99 PUR RPS EGFP | John L. Goodier | N/A |
| Plasmid: p99 JM111 EGFP | John L. Goodier | N/A |
| Plasmid: pL1RP-luc | Gerald Schumann | N/A |
| Software and Algorithms | ||
| BD FACSDiva™ Version 8.0 | BD Biosciences |
https://www.bdbiosciences.com RRID: SCR_001456 |
| Corel DRAW 2017 | Corel Corporation | https://www.coreldraw.com/ |
| GraphPad Prism Version 7 | GraphPad Software, Inc. |
https://www.graphpad.com RRID: SCR_002798 |
| ImageJ | Open source | http://imagej.nih.gov/ij/ |
| LI-COR Image Studio Lite Version 5.0 | LI-COR |
www.licor.com/ RRID: SCR_013715 |
| Other | ||
| Genomic Utility for Association and Viral Analyses in HIV | Open source | http://www.guavah.org/ |