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. Author manuscript; available in PMC: 2019 Aug 5.
Published in final edited form as: J Struct Biol. 2019 Mar 23;207(1):1–11. doi: 10.1016/j.jsb.2019.03.008

Fig. 2.

Fig. 2.

Graphical representation of key steps in segmentation pipeline. A) Classifier Selection: Three representative slices are taken from each of the principal axes. B) Classifier Generation: Each slice is manually classified based on the organelles within the volume. C) Volume Classification and Refinement: The classifiers are applied to the entire volume and produce segmented volumes of each class. The mitochondrial class is isolated, and each of the 3 volumes from the same axes are combined and non-overlapping data removed to produce an axial volume. D) Axial Volume Combination: Each of the refined volumes from the principal axes are combined, and non-overlapping data is removed.