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Effect of pH on the oxidative (grey bars) and reductive (black bars) activities of purified a) Sm7α‐HSDH, b) wt Cs7β‐HSDH, c) ADLN, d) ADDLN, and e) ADDAA variants of Cs7β‐HSDH and f) wt Ls7β‐HSDH. The activity at pH 8.0 was taken as 100 %. Enzymatic activities were determined by measuring NAD(P)+ reduction and NAD(P)H oxidation under the same conditions described in materials and methods. Values represent the mean of three independent experiments (mean±standard error).
