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. 2018 Nov 5;12(13):3192–3203. doi: 10.1002/cssc.201801862

Table 3.

Kinetic parameters of purified recombinant Sm7α‐HSDH.

k cat [c] K m K i k cat/K m
[s−1] [mmol L−1] [mmol L−1]
CA[a] 497.1 ± 17.2 0.960 ± 0.110 / 517.8 ± 77.2
CDCA[a] 873.1 ± 34.1 0.218 ± 0.024 11 ± 1.6 4005.2 ± 597.5
NAD+[b] 944.1 ± 9.1 0.560 ± 0.017 / 1685.8 ± 67.4

[a] The kinetic parameters were determined in the presence of 2.5 mm NAD+. [b] The kinetic parameters were determined in the presence of 2.0 mm CDCA. [c] k cat values were calculated considering a MW of 107.2 kDa.