Table 3.
k cat [c] | K m | K i | k cat/K m | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
[s−1] | [mmol L−1] | [mmol L−1] | |||||||||||||
CA[a] | 497.1 | ± | 17.2 | 0.960 | ± | 0.110 | / | 517.8 | ± | 77.2 | |||||
CDCA[a] | 873.1 | ± | 34.1 | 0.218 | ± | 0.024 | 11 | ± | 1.6 | 4005.2 | ± | 597.5 | |||
NAD+[b] | 944.1 | ± | 9.1 | 0.560 | ± | 0.017 | / | 1685.8 | ± | 67.4 |
[a] The kinetic parameters were determined in the presence of 2.5 mm NAD+. [b] The kinetic parameters were determined in the presence of 2.0 mm CDCA. [c] k cat values were calculated considering a MW of 107.2 kDa.