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. 2019 Aug 5;10:61. doi: 10.1186/s40104-019-0370-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Relative abundances of IZUMO1 as mean ± standard error of the mean (SEM) of GFE and PFE in pre-frozen (P-F) and frozen-thawed (F-T) boar spermatozoa. Values were normalized using α-tubulin protein as an internal standard. Each sperm sample were evaluated three times