. 2019 Aug 5;10:61. doi: 10.1186/s40104-019-0370-5

Table 1.

Sperm quality parameters in pre-frozen (P-F) and frozen–thawed (F-T) sperm, 30 (F-T-30 min) and 240 (F-T-240 min) min after thawing (mean ± SEM)

Parameter	Classification	P-F	F-T-30 min	F-T-240 min
% Total motile spermatozoa	GFE	72.52 ± 4.49^a,1	30.10 ± 1.91^a,2	8.14 ± 1.29^a,3
% Total motile spermatozoa	PFE	71.45 ± 6.53^a,1	4.45 ± 1.47^b,2	1.97 ± 0.34^b,2
% Progressive motile spermatozoa	GFE	54.25 ± 2.60^a,1	21.04 ± 1.77^a,2	5.02 ± 1.48^a,3
% Progressive motile spermatozoa	PFE	50.09 ± 5.01^a,1	1.83 ± 0.99^b,2	0.28 ± 0.09^b,2
% SYBR-14⁺ /PI⁻ spermatozoa	GFE	81.20 ± 1.65^a,1	28.77 ± 2.85^a,2	21.56 ± 3.46^a,3
% SYBR-14⁺ /PI⁻ spermatozoa	PFE	79.84 ± 3.42^a,1	5.60 ± 1.56^b,2	3.79 ± 1.01^b,2
% M540⁻/YO-PRO-1⁻ spermatozoa	GFE	75.13 ± 3.23^a,1	24.36 ± 2.33^a,2	14.59 ± 2.49^a,3
% M540⁻/YO-PRO-1⁻ spermatozoa	PFE	73.32 ± 3.58^a,1	6.26 ± 1.51^b,2	2.72 ± 0.72^b,2
% JC1_agg spermatozoa	GFE	79.14 ± 3.04^a,1	34.29 ± 2.36^a,2	27.57 ± 5.51^a,2
% JC1_agg spermatozoa	PFE	77.50 ± 3.28^a,1	16.50 ± 3.28^b,2	12.75 ± 5.88^a,2
% E⁻/YO-PRO-1⁻ spermatozoa	GFE	80.01 ± 2.23^a,1	21.92 ± 2.26^a,2	14.09 ± 3.15^a,3
% E⁻/YO-PRO-1⁻ spermatozoa	PFE	76.10 ± 2.79^a,1	8.86 ± 1.10^b,2	3.39 ± 1.82^b,2

Each ejaculate was classified as having good (GFE) or poor freezability (PFE). Different superscript numbers (^1,2,3) indicate significant differences (P < 0.05) between conditions (pre-frozen (P-F), F-T-30 min and F-T-240 min). Different superscript letters (^a,b) indicate significant differences(P < 0.05) between GFE and PFE in a given parameter