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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 May 22;177(1):29–40. doi: 10.1007/s10549-019-05279-9

Figure 3.

Figure 3.

Induction of NR4A1-dependent genes by DIM-C-pPhOH and buttressed analogs in breast cancer cells. (A) MDA-MB-231 and SKBR3 cells were treated with different concentrations of DIM-C-pPhOH (4-OH) for 24 h, and whole cell lysates were analyzed by western blots for induced expression of p21, SERPINB5 and GADD45α. MDA-MB-231 (B) and SKBR3 (C) cells were treated with 20 μM DIM-C-pPhOH (4-OH) or 5 μM of 9 buttressed analogs of DIM-C-pPhOH for 24 h, and whole cell lysates were analyzed by western blots for induction of p21, SERPINB5 and GADD45α. MDA-MB-231 (D) and SKBR3 (E) cells were treated for 24 h with different concentrations of 3 buttressed analogs of CIM-D-pPhOH containing 3-Cl, 3,5-Br2 and 3-Cl-5-OCH3 substituents, and whole cell lysates were analyzed by western blots for induction of p21, SERPINB5 and GADD45α.