Table 1.
Top significant pathways overrepresented among differentially expressed (DE) genes in response to in vitro exposure of bovine monocyte-derived macrophages (bMDM) to Streptococcus agalactiae strain ST103 or strain ST12, as compared to negative controls.
| Pathway name | P-value | Genes | ||
|---|---|---|---|---|
| ST103 vs control | Up-regulated (809) | Differential Regulation of Cytokine Production in Macrophages and T Helper Cells by IL-17A and IL-17F | 3,44E−11 | IL9, CSF2, CCL5, TNF, CCL2, IL17A, IL10, IL12B, CSF3, CCL4 |
| Granulocyte Adhesion and Diapedesis | 5,46E−10 | GNAI1, CCL8, CLDN4, CCL20, HRH1, CCL3L3, SDC1, IL1RN, TNF, ITGB3, CCL4, SDC3, IL33, CCL5, CXCL10, EZR, CXCL2, HSPB1, CCL22, CCL2, CSF3, SDC4, ICAM1, IL36G, PECAM1 | ||
| Differential Regulation of Cytokine Production in Intestinal Epithelial Cells by IL-17A and IL-17F | 7,78E−10 | IL9, CSF2, CCL5, TNF, CCL2, IL17A, IL10, IL12B, CSF3, CCL4 | ||
| Down-regulated (882) | IL-8 Signaling | 9,28E−08 | PLCB2, GNG7, PLD1, ARRB2, IRAK3, VCAM1, NCF2, FOS, PIK3CD, RND3, VEGFC, PRKCE, LIMK2, ITGB5, ANGPT2, FLT4, PRKD3, PLD4, MYL9, PIK3CG, PIK3R2, CCND1, CXCR2, PLD2, CYBB | |
| Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses | 3,49E−06 | PRKCE, OAS1, IL12A, TLR7, PRKD3, NOD1, OAS2, C3AR1, C1QA, PIK3CG, C1QC, IL18, PIK3CD, TLR3, PIK3R2, C5AR1, CLEC6A, CLEC7A | ||
| Phagosome Formation | 2,76E−05 | PRKCE, TLR7, PRKD3, PLCB2, MRC2, MSR1, C3AR1, PIK3CG, INPP5D, PIK3CD, TLR3, PIK3R2, C5AR1, ITGA4, RND3, CLEC7A | ||
| ST12 vs control | Up-regulated (934) | Th1 and Th2 Activation Pathway | 9,08E−12 | PRKCQ, JAK3, IL12RB2, GAB1, CD274, IL27, DLL4, IL9, STAT1, CD3D, STAT5A, IL12B, CRLF2, NFIL3, IL33, IL27RA, S1PR1, CD3E, STAT4, PIK3R3, JAG1, LTA, IL2RA, HLA-DOB, NFATC1, TNFSF4, DLL1, IL10, ICAM1, TBX21 |
| Differential Regulation of Cytokine Production in Macrophages and T Helper Cells by IL-17A and IL-17F | 1,52E−10 | CSF2, IL9, CCL5, TNF, CCL2, IL17A, IL12B, IL10, CSF3, CCL4 | ||
| Role of Hypercytokinemia/Hyperchemokinemia in the Pathogenesis of Influenza | 2,88E−09 | CCL5, CXCL10, IL37, CXCL8, IL9, IL1RN, TNF, CCL2, IL17A, IL12B, CCL4, IL36G, IL33 | ||
| Down-regulated (845) | IL-8 Signaling | 5,55E−05 | ITGB5, LIMK2, ANGPT2, FLT4, PLCB2, GNG7, MYL9, PLD4, PLD1, ARRB2, VCAM1, NCF2, FOS, PIK3CD, PIK3R2, CCND1, CXCR2, PLD2, RND3 | |
| Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses | 7,97E−05 | OAS1, TLR7, C1QB, NOD1, OAS2, C3AR1, C1QA, C1QC, IL18, PIK3CD, TLR3, C5AR1, PIK3R2, CLEC6A, CLEC7A | ||
| Complement System | 2,36E−04 | C1QC, C5AR1, C1QB, C7, CFD, C3AR1, C1QA | ||
| ST12 vs ST103 | Up-regulated (42) | IL-12 Signaling and Production in Macrophages | 1.30E−04 | FOS, REL, PIK3R3, ALOX15 |
| Prolactin Signaling | 4.36E−04 | FOS, PIK3R3, TCF7 | ||
| Rac Signaling | 1.18E−03 | PIK3R3, CYFIP2, ABI2 | ||
| Down-regulated (18) | Thiosulfate Disproportionation III (Rhodanese) | 1.78E−03 | TST | |
| Th2 Pathway | 3.46E−03 | TNFSF4, IL2RA | ||
| Granulocyte Adhesion and Diapedesis | 4.89E−03 | CCL8, CLDN4 | ||
The number in parentheses represents the number of DE genes used for Ingenuity Pathway Analyses (IPA).