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. 2019 Jun 24;116(31):15475–15484. doi: 10.1073/pnas.1905488116

Fig. 4.

Fig. 4.

Rearrangement of E1 cross-over and reentry loops connecting SCCH and AAD domains. (A) Cartoon representations of UAE in the open and closed conformations, with Ub in ribbon and ATP or AVSN in stick, showing locations of cross-over and reentry loops in blue ribbon. Helices H14 and H27 are shown in orange for each structure to illustrate movement of the SCCH domain. (B) Ribbon representations of the cross-over and reentry loops of Uba1 in the open (Left) and closed (Right) conformations. Ub is displayed in yellow. Active site cysteine residues are colored green, with the nucleotide labeled and shown in stick representation. Orientation is as in A. (C) Cross-over and reentry loops of Uba1 (blue) and SAE (red) superimposed in the open (Left) and closed (Right) conformations. Active site cysteine residues colored green. Uba1 open conformation: PDB ID code 4II3; Uba1 closed conformation: this work; SAE open conformation: PDB ID code 1Y8R; SAE closed conformation: PDB ID code 3KYD. Color scheme for domains is as described for Fig. 2.