Fig. 3.

BOK is a marker of chemoresistance. (A) Analysis of various 5-FU sensitive (P or parental) and resistant (R) colorectal cancer cell lines (developed in vitro) for BOK protein status by Western blot (Left) and the Bok mRNA status by droplet digital PCR (Right). (B) Analysis of 5-FU-sensitive and 5-FU-resistant primary colorectal cancer tissues for BOK protein levels. WT and Bok^−/− HEPG2 cells were used as the antibody control. (C) Analysis of 5-FU-sensitive and 5-FU-resistant colorectal cancer organoids for BOK protein levels. WT and Bok^−/− HCT15 cells were used as the antibody control. Error bars ± SEM (n = 3), *P ≤ 0.005.