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. 2019 Jul 16;116(31):15469–15474. doi: 10.1073/pnas.1904523116

Fig. 4.

Fig. 4.

5-FU-mediated p53 induction and genotoxic stress is BOK-dependent. (A) MEFs of various genotypes (as indicated) expressing a p53-GFP reporter were treated with either 5-FU (50 µg/mL) or etoposide (1 µg/mL), and the readout (GFP induction) was measured 24 h later by FACS analysis. (B) Basal levels of p53 were measured by the p53 reporter assay in MEFs (Left), RNA analysis in mouse liver samples (Right), and Western blot analysis (Bottom) in WT and their Bok−/− counterparts. (C) The increase in the basal level of p53 in Bok−/− HCT116 cells could be reversed by culturing the cells in UMP and CMP (1 mM). Nonspecific band (NS) is used as loading control. (D) The p53 transcriptional target p21 induction could be reversed by culturing the cells in UMP and CMP (1 mM). Error bars ± SD, n = 3 (except for D, where error bars ± SEM, n = 3). *P ≤ 0.005.