Fig. 1.

Presence of CcrM during the swarmer cell cycle and the stalked cell cycle. Each cell division is asymmetric yielding a SW cell and a ST cell. Circles and theta structures within the cells (red) indicate the single circular chromosome. The swarmer cell chromosome can only initiate replication once it has differentiated into a stalked cell, whereas the chromosome in the stalked cell that arises from a cell division can immediately initiate replication. Shown are immunoblots of protein samples from synchronized wild-type cultures using anti-CcrM antibody. Isolated swarmer cells were incubated in M2G minimal media and allowed to progress through the cell cycle. Upon cell division, the culture was subjected to a second synchronization. The swarmer and stalked cell fractions collected from the second synchronization were released in M2G for swarmer and stalked cell cycle analyses, respectively.