Fig. 1.

Deletion of NMIIA and NMIIB in GBM increases survival and reduces proliferation. (A) Mouse model of GBM. Reprinted with permission, Cleveland Clinic Center for Medical Art & Photography © 2019. (B) Kaplan–Meier curves comparing the survival of retroviral-induced GBMs in mice with floxed alleles for PTEN (n = 9) and for NMIIA, NMIIB, and PTEN (n = 16). The significance of differences in survival were assessed by both log-rank (Mantel–Cox) test and Gehan–Breslow–Wilcoxon tests and both give a P value of <0.0001. (C) Western blot demonstrating depletion of NMIIB heavy chain isoform using shRNA lentivirus from glioma cell lines genetically deleted for NMIIA with PDGF-IRES-cre retrovirus. (D) Nuclear doubling rate as measured by nuclear count every 3 h for 5 d, using Histone 2B-RFP–expressing glioma cells (n = 3). Statistical significance was assessed with a two-tailed t test.