Fig. 3.

NFPs regulate transmural growth and cardiomyocyte proliferation autonomously. (A) Control hearts and (B) iDKO hearts show sporadic clones labeled with different colors. Cardiomyocytes in hearts were induced to be labeled at E7.75 with dosage of tamoxifen at 20 μg/g body weight and the hearts at E12.5 were imaged via Leica M205 FA, which can detect fluorescent protein-labeled cells at single-cell resolution. (C–E) Individual clones in the cleared hearts were 3D-imaged to identify the geometric pattern of each of the clones. C, D, and E show compact, trabecular and transmural clones, respectively. (F) The clonal patterns between control and iDKO. (G) The transmural growth of the clones and that the iDKO clones grow significantly shorter distances compared with the control clones. (H) The number of cells in each clone; the iDKO clones contain significantly more cells than the control clones across all 3 types of clones. (Scale bars in A and B: 200 μm and in C–E: 50 μm.)