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. 2019 Jul 16;116(31):15453–15462. doi: 10.1073/pnas.1903949116

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Fig. 2. — Overview of the imaging phospholipase D (PLD) activity with clickable alcohols via transphosphatidylation (IMPACT) method for visualizing PLD activity in live cells. (A) Cartoon schematic of IMPACT. (B) Comparison of established IMPACT method using azido alcohols and strain-promoted azide–alkyne cycloaddition (SPAAC) detection with the advance reported here, real-time (RT) IMPACT, using trans-cyclooctene (TCO)-containing alcohols and inverse electron-demand Diels–Alder (IEDDA) detection using tetrazine (Tz) reagents. (C) Structures of trans-5-oxocene (oxoTCO) alcohols and the fluorogenic Tz–BODIPY conjugate used in this study.