Fig. 4.

Nucleotide neighbor-joining tree for phased haplotypes of gene 8960. Each NMP clone was phased into 2 sequences, the marker-linked (NMP-associated) haplotype (solid red circle) and the nonmarker haplotype (hollow red circle). MP clones are not phased and have only one sequence (black circle). D. pulicaria clones are marked with blue circles. Clones from each population are color coded by their names (KAP: black; NFL: green; POV: blue; LPB: orange; TEX: pink). The tree is rooted by D. obtusa. Five hundred bootstraps were run, and bootstrap values ≥70% are shown. The tree was constructed using the neighbor-joining method incorporated into MEGA7 (49), and pairwise deletion was applied to deal with gaps and missing data.