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Nucleotide neighbor-joining tree for complete mitochondrial sequences from 5 populations of D. pulex, along with sequences from D. pulicaria isolates. Five hundred bootstraps were performed and bootstrap values ≥70% are shown. MP: male-producing clones (black circles); NMP: nonmale-producing clones (red circles); D. pulicaria sequences are marked with blue circles. Clones from each population are color coded by their names (KAP: black; NFL: green; POV: blue; LPB: orange; TEX: pink).
