Fig. 1.

CYP71A27 is connected to camalexin synthesis. (A) Col-0, cyp71A12, cyp71A13, cyp71A27, and cyp71A28 plants were grown for 2 wk in soil (10%)/sand mixture; afterward, 2 soil samples were taken per plant, and sulfatase activity was measured. Data are presented as means and SE from 10 samples corresponding to 5 independent plants. Asterisks indicate significant differences from the wild-type Col-0 significant at P < 0.05 (Student’s t test). (B) Camalexin accumulation in roots of plants grown in soil (10%)/sand mixture like for sulfatase activity. Data are presented as means and SD from 4 pools of at least 5 independent plants. Asterisks indicate significant differences from Col-0 significant at P < 0.05 (Student’s t test). (C) Col-0 and the 4 mutant lines were grown for 2 wk in presence of Pseudomonas sp. CH267 or 10 µM MgCl₂ as mock, and the FW of whole plants was measured. Data are presented as means and SE from at least 20 plants grown on 4 independent plates. Asterisks indicate significant differences between mock and bacterial treatment at P < 0.05 (Student’s t test).