Fig. 4.

CrkII-binding motif is insufficient for PEAK3-dependent inhibition of CrkII. (A) Schematic representation of used PEAK3 constructs. (B) Average perimeter of COS-7 cells transiently expressing either wild-type or the ΔPK variants of PEAK3 with an empty vector or untagged CrkII. Data represent the mean ± SEM of 3 independent experiments (n = 20 cells in each experiment), ***P < 0.001. (C) Coimmunoprecipitation of FLAG-tagged wild-type and ΔPK PEAK3 variants, transiently expressed in HEK293 cells, with exogenously expressed, untagged CrkII. Protein levels were detected with the indicated antibodies by Western blot. Data are representative of 3 independent experiments.