Figure 1.

miR-221 was upregulated in LPS-induced ALI in mice. Mice (n = 3 each) were treated with a single dose of LPS (2 mg/kg) or normal saline, and total RNAs were isolated from their lungs at 24 h after the treatment. (a) Heat map of miRNA profiles represented the significantly regulated miRNAs (n = 3/group). (b-d) miR-221 expression was validated by qRT-PCR in lung tissues, peripheral blood and splenocytes of mice challenged with LPS (n = 6/group). Data represent the mean ± SD of three independent experiments. **p < 0.01 vs. control group.