Figure 2.

The effects of abnormally expressed miR-221 on LPS-induced injury. Mice were injected intravenously (tail vein) with agomir-221, antagomir-221, control antagomir or control agomir (2 mg/kg) 24 h prior to 2 mg/kg LPS treatment. The mice were sacrificed after LPS administration for 24 h and then lung tissues were collected for analysis. (a) The efficiency of agomir-221 or antagomiR-221 was determined by qRT-PCR. (b) The Kaplan–Meier method was used to compare the survival rates among different groups (n = 10/group). (c) Lung permeability was assessed using the Evans blue dye extravasation method (n = 6/group). (d) The pulmonary edema was evaluated by the lung wet/dry weight ratio (n = 6/group). (e) The degree of injury of lung samples was assessed with H&E staining at ×400 magnification (n = 6/group). *p < 0.05, **p < 0.01 vs. control group. ##p < 0.01 vs. LPS alone group.